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Published Research Using the Platform

Izon's qEV isolation and TRPS/NPS single-particle measurement technology is cited in a continually growing body of peer-reviewed research — from extracellular vesicle biomarker discovery to cancer diagnostics, cardiovascular biology, and infectious disease. A curated selection is below.

1000+
Publications citing Izon's qEV & TRPS/NPS platform
Nature · PNAS · JEV
Published in leading peer-reviewed journals
EVs · Viruses · LNPs
Spanning isolation, characterisation & bioprocessing

Figures reflect Izon Science's global publications database. See izon.com/publications for the complete, continually updated list.

Landmark Research

The Foundational & Highest-Profile Studies Behind the Platform

The papers that established qEV and TRPS as validated methods in the field, alongside recent work from Harvard Medical School's research centres.

Journal of Extracellular Vesicles · 2014 1,000+ citations

Single-step isolation of extracellular vesicles by size-exclusion chromatography

The original single-step SEC protocol for isolating EVs from body fluids — the method Izon's qEV column directly commercializes. One of the most-cited papers in the EV isolation field. (Amsterdam UMC)

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Journal of Extracellular Vesicles · 2014 Foundational TRPS study

Reproducible extracellular vesicle size and concentration determination with tunable resistive pulse sensing

Established TRPS as a reproducible way to size and count individual EVs, resolving the pore-to-pore variability that had limited earlier single-particle methods. (Amsterdam UMC)

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Journal of Extracellular Vesicles · 2016 6-lab international study

A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing

Six independent labs across four countries jointly validated a standardized TRPS protocol for EV concentration measurement, using qEV-isolated plasma EVs as the reference material. (University of Queensland, Duke University, Erasmus MC, Aarhus University, La Trobe University)

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Journal of Extracellular Vesicles · 2018 ISEV consensus guideline

MISEV2018: Minimal information for studies of extracellular vesicles

The International Society for Extracellular Vesicles' formal position statement on best-practice EV research, naming SEC (qEV) and TRPS among the accepted isolation and measurement methods. One of the most-cited papers in EV science.

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Preprint · 2025 Harvard Medical School

Exploring size exclusion chromatography columns 20 and 35 nm pore size effect for isolation of extracellular vesicles

Compares 20 nm and 35 nm qEV pore sizes for EV yield and purity, from the Cardiovascular Research Center at Massachusetts General Hospital, Harvard Medical School, with Johns Hopkins and Drexel University co-authors.

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Journal of Extracellular Biology · 2024 Harvard Medical School

Multimode chromatography-based techniques for high purity isolation of extracellular vesicles from human blood plasma

Evaluates multimode chromatography techniques for high-purity EV isolation from human plasma, from Harvard Medical School's Nano Flow Core Facility at Beth Israel Deaconess Medical Center.

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Molecular Therapy · 2021 Cell Press journal

A SARS-CoV-2 targeted siRNA-nanoparticle therapy for COVID-19

Uses TRPS to characterize an LNP-delivered siRNA therapeutic candidate against SARS-CoV-2, published in the high-impact gene- and cell-therapy journal Molecular Therapy. (Griffith University, Australia)

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Journal of Proteome Research · 2025 9-method benchmark

Comprehensive comparison of methods for isolation of extracellular vesicles from human plasma

Benchmarks qEV columns against eight other EV isolation methods for proteomic yield and purity from just 100 µL of human plasma. (University of North Carolina at Greensboro)

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J. Controlled Release · 2020 CNRS / INSERM

Theranostic MRI liposomes for magnetic targeting and ultrasound-triggered release of the antivascular CA4P

Uses TRPS to characterize magnetically targeted, ultrasound-triggered liposomes carrying an anti-vascular cancer drug, developed by CNRS and INSERM's Paris research consortium.

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LNP & VLP Research

Backing the Fastest-Growing Segments of Nanomedicine

Lipid nanoparticles and virus-like particles now represent a larger and faster-growing market than extracellular vesicles alone — spanning mRNA vaccines, gene editing, gene therapy, and next-generation vaccine platforms.

Nano Letters · 2023 MIT

In vivo RNA delivery to hematopoietic stem and progenitor cells via targeted lipid nanoparticles

An antibody-targeted LNP, purified using qEV columns, delivers RNA and mRNA directly to blood stem cells in living rodents — achieving gene editing in roughly 90% of hematopoietic stem and progenitor cells from a single injection. (MIT, Koch Institute for Integrative Cancer Research)

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Allergy · 2021 Phase I clinical trial

Development and preclinical evaluation of a virus-like particle vaccine against COVID-19

TRPS confirmed that a multi-antigen VLP vaccine candidate matched the size of the authentic SARS-CoV-2 virion. The candidate has since advanced into a Phase I clinical trial (NCT04818281).

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Molecular Therapy · 2021 Cell Press journal

A SARS-CoV-2 targeted siRNA-nanoparticle therapy for COVID-19

Uses TRPS to characterize an LNP-delivered siRNA therapeutic candidate against SARS-CoV-2, published in the high-impact gene- and cell-therapy journal Molecular Therapy. (Griffith University, Australia)

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Frontiers in Bioeng. & Biotech. · 2022 Vaccine process monitoring

Bioanalytics for influenza virus-like particle characterization and process monitoring

TRPS was used alongside other biophysical methods across six Influenza VLP variants (groups A and B, monovalent and pentavalent) to monitor consistency during vaccine manufacturing. (iBET, Portugal)

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Biotechnology & Bioengineering · 2021 Gene therapy manufacturing

Oncolytic virus purification with periodic counter-current chromatography

TRPS measured particle-size distribution to guide continuous-chromatography purification of an oncolytic adenovirus, improving productivity and robustness for gene-therapy manufacturing.

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J. Molecular Liquids · 2024 Liposome drug delivery

Long-term shelf-life liposomes for delivery of prednisolone and budesonide

Uses qEV columns to separate drug-loaded liposomes from free drug, assessing the entrapment capacity and shelf-life stability of steroid-carrying liposomes for anti-inflammatory delivery.

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J. Controlled Release · 2020 CNRS / INSERM

Theranostic MRI liposomes for magnetic targeting and ultrasound-triggered release of the antivascular CA4P

Uses TRPS to characterize magnetically targeted, ultrasound-triggered liposomes carrying an anti-vascular cancer drug, developed by CNRS and INSERM's Paris research consortium.

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Journal of Extracellular Vesicles · 2020 Virus-EV separation

Efficient and reproducible depletion of hepatitis B virus from plasma-derived extracellular vesicles

Used qEV isolation to remove unbound antibody and the majority of Hepatitis B virions from plasma, opening the door to functional studies that separate EVs from co-isolated virus particles.

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Isolation & Methodology

Validating Size-Exclusion Chromatography for EV Isolation

Method-comparison studies that validate SEC as a reliable, reproducible way to isolate EVs — the groundwork every application-specific study downstream depends on.

BMC Methods · 2024 SEC vs. 3 other methods

Confirming size-exclusion chromatography as a clinically relevant EV separation method from 1 mL plasma

Benchmarks SEC against ultracentrifugation, precipitation, and immunoaffinity capture from a clinically realistic 1 mL plasma volume — finding SEC delivers the best combination of particle yield, purity, and downstream proteomic usability.

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EVCNA · 2024 10-column comparison

Choice of size-exclusion chromatography column affects recovery, purity and miRNA cargo analysis of EVs from human plasma

Compares recovery and separation efficiency across ten different SEC columns, showing resin pore size is a key variable that downstream miRNA biomarker studies need to control for.

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Int. J. Molecular Sciences · 2024 Multiple myeloma

Size-exclusion chromatography: a path to higher yield and reproducibility vs. sucrose cushion ultracentrifugation for EV isolation in multiple myeloma

Directly compares UF-SEC with sucrose-cushion ultracentrifugation across three myeloma cell lines and patient plasma, reporting more consistent, higher-yield EV recovery with SEC.

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Bioprocessing & MSC-EV

Scaling EV Isolation from Bench to Manufacturing

As EV- and MSC-EV-based therapeutics move toward clinical manufacturing, isolation methods need to scale without sacrificing purity or function — an area qEV columns are increasingly benchmarked against.

J. Dairy Science · 2021 Large-volume biofluid

Comparison of isolation methods using commercially available kits for obtaining extracellular vesicles from cow milk

Benchmarks qEV against other isolation kits for extracting EVs from cow milk — a large-volume, industrially relevant biofluid outside the plasma and urine samples most isolation studies focus on.

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Journal of Extracellular Vesicles · 2021 5-method comparison

Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

Compares five isolation methods, including qEV, across recovery, purity, and subcellular origin — helping manufacturers match the right isolation approach to the intended clinical application.

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Nanomedicine: NBM · 2015 Karolinska Institutet

Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

A foundational scale-up study combining ultrafiltration with SEC for high-yield EV isolation that preserves biophysical and functional properties — still widely cited in manufacturing protocols today.

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Nanomedicine: NBM · 2017 SEC vs. ultracentrifugation

Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation

Directly compares SEC- and ultracentrifugation-isolated EVs, showing SEC preserves EV functional activity better — key evidence for manufacturers replacing ultracentrifugation with column-based isolation at scale.

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J. Cellular & Molecular Medicine · 2021 hADSC (MSC-type) EVs

Identification and characterization of hADSC-derived exosome proteins from different isolation methods

Uses Izon qEV10 columns to isolate exosomes from human adipose-derived stem cells (hADSC) and compares the resulting protein cargo across isolation methods.

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Eur. J. Pharm. & Biopharm. · 2022 University of Navarra

Isolation methods of large and small extracellular vesicles derived from cardiovascular progenitors: A comparative study

Uses Izon qEV10/35 nm columns to compare large- and small-EV isolation from cardiac progenitor cells, informing isolation choices for cardiac regenerative applications.

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Regenerative Medicine

EV-Based Approaches to Tissue Repair

From cartilage regeneration to wound healing, EV-based regenerative approaches are an emerging application area — including some of the newest work on plant-derived extracellular vesicles.

Int. J. Molecular Sciences · 2025 University College London

Isolation, characterization, and in vitro cell studies of plant-based exosome-like nanovesicles for treatment of early osteoarthritis

Uses a 35 nm qEV column to isolate grapefruit-derived exosome-like nanovesicles, evaluated as a candidate cartilage-regenerative treatment for early-stage osteoarthritis. (UCL, Royal National Orthopaedic Hospital)

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ACS Biomaterials Sci. & Eng. · 2025 4-method comparison

Optimizing isolation methods and exploring the therapeutic potential of lotus-derived extracellular vesicles in modulating inflammation and promoting wound healing

Directly benchmarks qEV alongside tangential flow filtration, ultracentrifugation, and density-gradient ultracentrifugation for isolating lotus-derived EVs used in a wound-healing model. Tangential flow filtration gave the highest purity and yield in this particular study — an honest data point on when qEV is, and isn't, the top performer. (National Taiwan University)

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Oncology & Cancer Diagnostics

EV-Based Liquid Biopsy & Cancer Biology

EVs are emerging as liquid-biopsy biomarkers across cancer types — from blood-based screening tests to mechanistic studies of how tumors use EVs to spread.

Journal of Extracellular Vesicles · 2024 IVDR-track biomarker

Towards IVDR compliance in a quantitative EV-miRNA liquid biopsy assay for classic Hodgkin lymphoma

Adds quality-control steps — including SEC-based EV isolation — to an miRNA liquid biopsy assay, moving a Hodgkin lymphoma treatment-response test closer to regulatory-grade clinical use.

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Journal of Molecular Diagnostics · 2024 97% sensitivity/specificity

Improving specificity for ovarian cancer screening using a novel extracellular vesicle-based blood test

Reports a tumour-EV blood test achieving 97% specificity and 97% sensitivity for high-grade serous carcinoma in an independent, blinded verification cohort of nearly 400 women.

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Nature Communications · 2024 Liver metastasis mechanism

Tumor-derived miR-9-5p-loaded EVs regulate cholesterol homeostasis to promote breast cancer liver metastasis

Identifies an exosomal microRNA mechanism that reprograms liver cholesterol metabolism to support breast cancer metastasis, with antagomir treatment shown to block spread in a mouse model.

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Cancer Discovery · 2024 Brain organoid model

Glioblastoma cortical organoids recapitulate cell-state heterogeneity and intercellular transfer

Uses long-term cultured brain organoids to show glioblastoma cells transfer material to healthy neural cells via extracellular vesicles — a mechanism that may help reprogram the tumour microenvironment.

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Neurodegenerative & Cardiovascular Disease

EV Biomarkers Beyond Oncology

EV biomarkers extend well beyond cancer, offering minimally invasive windows into neurodegenerative disease progression and vascular health.

Nature Medicine · 2024 704-patient cohort

Plasma extracellular vesicle tau and TDP-43 as diagnostic biomarkers in FTD and ALS

Across a 704-patient cohort, plasma EV tau and TDP-43 levels distinguish frontotemporal dementia and ALS subtypes with high accuracy — a minimally invasive alternative to current diagnostic pathways.

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Journal of Extracellular Vesicles · 2024 Vascular disease mechanism

Low fluid shear stress stimulates the uptake of noxious endothelial EVs via MCAM and PECAM-1

Shows blood-vessel regions exposed to low shear stress — where atherosclerotic plaques typically form — take up far more endothelial EVs, pointing to an EV-driven mechanism in early vascular disease.

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Journal of Extracellular Vesicles · 2024 Cell-free therapy candidate

Adult cardiomyocyte-derived EVs for the treatment of cardiac fibrosis

Shows EVs from healthy adult heart-muscle cells reduce fibrotic markers and improve heart function when injected into an animal model of cardiac fibrosis — a candidate cell-free therapy.

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Infectious Disease

EVs in Viral Infection & Host Response

Extracellular vesicles both reveal how the body responds to infection and, in some cases, carry the virus particles themselves — two distinct angles qEV isolation supports.

Journal of Extracellular Vesicles · 2024 39-patient cohort

EVs containing SARS-CoV-2 proteins are associated with multi-organ dysfunction and worse outcomes in severe COVID-19

Tracks EV spike and nucleocapsid protein levels across 39 severe COVID-19 patients over time, linking higher EV viral-protein expression to poorer antibody response and worse clinical outcomes.

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Journal of Extracellular Vesicles · 2020 Virus-EV separation

Efficient and reproducible depletion of hepatitis B virus from plasma-derived extracellular vesicles

Used qEV isolation to remove unbound antibody and the majority of Hepatitis B virions from plasma, opening the door to functional studies that separate EVs from co-isolated virus particles.

Read the paper →

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